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. 2018 Jan 11;8:545. doi: 10.1038/s41598-017-19071-6

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© The Author(s) 2018

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Effects of caveolin-1 deficiency on epithelial parameters by immunoblotting. (a) Representative immunoblots of WT (n = 6) and Cav1-deficient (Cav1−/−; n = 6) kidney lysates detected by antibodies to Cav1, alpha subunit of Na/K-ATPase (Na⁺/K⁺), NKCC1/2 (antibody recognizes both NKCC isoforms), AQP1, V2R, NKCC2, phosphorylated (p) NKCC2 (pT96/T101-NKCC2), NCC, pNCC (pS71-NCC), AQP2, pAQP2 (pS256-AQP2), alpha subunit of epithelial sodium channel (ENaCα) and aquaporin 4 (AQP4); β-actin serves as loading control below the respective blots; all molecular weight levels are approximate. (b) Densitometric quantification of the immunoreactive signals normalized to the respective loading controls. Data is expressed as the mean ± standard deviation; *p < 0.05 (Student’s t test for normal distribution); original immunoblot scans are available in Supplementary Data.