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. 2018 Jan 11;8:478. doi: 10.1038/s41598-017-18744-6

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© The Author(s) 2017

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Materials for additive manufacturing of artificial axons (left, Young’s elastic modulus < 10⁷ Pa) afford mechanical stiffness more similar to CNS cells and tissues than materials used currently for myelination assays (right, Young’s elastic modulus >10⁷ Pa); *reported literature values from refs^{14–16,36–39,44}. The Young’s elastic moduli of artificial axon materials were determined by means of atomic force microscope-enabled nanoindentation (see Materials and Methods).