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. 2017 Nov 14;118(1):52–61. doi: 10.1038/bjc.2017.388

Figure 4.

Figure 4

BET and YAP1 inhibitors blocked GC cell proliferation, invasion and migration induced by Gal-3. (A) Cell growth of Snu1 and GT5 cells in which over-expressed by Gal-3 cDNA or Vector (V) treated with JQ1 (A) or CA3 (B) as indicated dosage was determined by using the CellTiter Aqueous One Solution Cell Proliferation Assay kit as described in Materials and Methods, **P<0.01; ***P<0.001. (B) Cell growth of Snu1 and GT5 cells in which over-expressed by Gal-3 cDNA or Vector (V) treated with JQ1 (1, 5 μM) or CA3 (0.5 μM) alone or the combination of CA3 with JQ1 as indicated dosage was determined using MTS. (C). Invasion assay was performed in Snu1 and GT5 with Gal-3 stably transfection and treated with CA3 and JQ1 as described in Materials and Methods; representative fields (top panel) and quantification (low panel) are shown. (D). Migration assay was performed in Snu1 and GT5 with Gal-3 stably transfection and treated with CA3 and JQ1 as described in Materials and Methods; Representative fields of migration of GAC cell with or without Gal-3 over-expression are shown.