Fig. 1.

Dynamic explant culture setup. A) A polyurethane foam support was used to support the hindlimb explants during culture. The rectangular piece of foam was cut to create a step along the horizontal axis. Measurements are in mm. B) Explants were positioned onto the lower level of the foam and pinned into place. Explants were orientated medial side down with the hip joint furthest away from the step. C) Uniaxial compression of the foam support caused the hindlimb to bend at the knee joint mimicking a flexion motion of the knee joint.