Figure 1.

Schematic diagram of reporter hepatitis B virus generated in this study. pgRNA contains 3363 nucleotides. Nucleotide sequences of seven bases at both ends in pgRNA are shown. Open reading frames shown with blue rectangles indicate nucleotide numbers of the initiation and the C‐terminal in each protein. Red rectangles indicate the NanoLuc gene or secNL gene with several flanking nucleotide sequences. GLuc gene containing DNA is shown as a green rectangle. Dotted lines indicate deleted sequences in the pgRNA. Insertion site of the reporter genes is between 223 and 224 of pgRNA. Nucleotide numbers of the 5′ and 3′ end of the deleted sequences in pgRNA are also shown. Numbers in italics represent the DNA size of the inserted genes. Numbers on the right indicate the size of the recombinant pgRNA