Figure 5.
Comparative analysis of the RNA level of reporter virus in cells infected with hepatitis B virus/NanoLuc (HBV/NL), HBV/secNL and HBV/GLuc as well as analysis of the correlation of reporter activity with virus RNA. (A) Relative HBV RNA level in cells infected with HBV/NL, HBV/secNL and HBV/GLuc. Recombinant viruses equivalent to 100 DNA copies per cell were infected to 1 × 106 HepG2/NTCP#22 cells. RNA in cell lysates after 6 days of infection were analyzed for HBV RNA by quantitative RT‐PCR. (B,C) Time course‐dependent measurement of the relative amount of reporter activity and RNA of the reporter HBV in infected cells. One hundred copies of HBV/secNL (B) or HBV/GLuc (C) per cell were used to infect 1 × 106 HepG2/NTCP#22 cells. At the indicated days, culture medium of HBV/secNL infected cells or HBV/GLuc infected cells were measured for reporter activity. Simultaneously, virus RNA in a 1/10 volume of cell lysate was quantitated by RT‐PCR using the primer set described in the Materials and Methods