Figure 8.
Trans‐rescue experiment of reporter hepatitis B virus (HBV) production. Reporter viruses were harvested from HepG2 transfected with the indicated plasmids together with pHBV/D. The viruses were used to infect 2 × 105 PXB cells in the presence or absence of 100 copies per cell of HBV obtained from primary human hepatocytes (PHH) maintained in urokinase‐type plasminogen activator transgenic/SCID mice. Virus was prepared from the medium and used to infect 1 × 105 HuH7/NTCP cells. NanoLuc (NL) activity was measured at the indicated times. On day 16, an aliquot of the cells was treated with 80 nmol/L entecavir. Solid lines indicate relative NL activity in HuH7/NTCP infected with culture medium recovered from PXB cells infected with HBV/NL (red), HBV/NLS+pol (green) and HBV/NLS+polS (blue) together with wild‐type HBV. Dotted lines indicate relative NL activity in cells infected with culture medium recovered from PXB cells infected with HBV/NL (red), HBV/NLS+pol (green) and HBV/NLS+polS (blue) without wild‐type HBV. Double lines with red (HBV/NL) and with green (HBV/NLS+pol) show NL activity after entecavir treatment. Experiments were conducted twice and the mean value is plotted in this figure