Figure 1.

Functions and tissue-specificity of DBL-1 signaling in lipid storage. (A) Both dbl-1 and lon-2 mutants show a decrease in lipid levels via Oil Red O staining, similar to that of fat mutants. Animals were grown at 20° and stained at the L4 larval stage. Quantification was done for equivalent regions of the intestine just posterior to the pharynx. (B) Images of L4 animals stained with Oil Red O taken at 400×. (C) Loss of sma-3 results in decreased lipid levels. Expression of sma-3 in the hypodermis is sufficient to rescue the lipid phenotype. Expression in either the pharynx or the intestine cannot rescue lipids to wild-type levels. Animals were grown at 20° and stained at the L4 larval stage. Quantification was done for equivalent regions of the intestine just posterior to the pharynx. (D) Images of L4 animals stained with Oil Red O taken at 400×. Anterior is to the left. For all graphs, asterisks across the bottom denote significance compared to control: n.s., not significant; *** P value < 0.001. Error bars denote SEM.