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. 2017 Oct 9;153(2):214–224. doi: 10.1111/imm.12834

Figure 5.

Figure 5

Functional characterization of OT2 follicular helper T (Tfh) ‐like cells. Untouched CD4+ T cells from naive OT2 mice were co‐cultured with freshly isolated b12 B cells and wild‐type (wt) dendritic cells (DCs) in the presence of different virus‐like particles (VLPs). (a, b) Histograms represent intracellular expression of interleukin‐21 (IL‐21) (a) and IL‐4 (b) among subpopulations of OT2 CD4+ T cells from co‐cultures in the presence of Env‐OT2‐VLPs. Numbers specify geometric fluorescent mean of corresponding cell populations. The data of one representative experiment out of four are shown; the gating strategy is shown in the Supplementary material (Fig. S3). (c) RT‐qPCR analysis of IL‐21 mRNA in the bulk RNA on days 4, 5 and 6 of the culturing with Env‐OT2‐VLPs. Expression of IL‐21 is shown as the fold increase over unstimulated controls after normalization to housekeeping genes. The histograms represent the mean values from three independent experiments ± standard deviation. (d) Supernatants from 6‐day cultures were analysed by ELISA for anti‐Env IgG1. The diagrams represent the mean values from seven independent experiments ± standard deviation. *p < 0·05; **p < 0·01; ***p < 0·001 one‐way analysis of variance with Tukey's post test.