Figure 1.

IL‐33 elicits airway, blood and bone marrow eosinophilia. (a) Experimental model of IL‐33‐induced inflammation. (b) Contrast‐stained cells; arrows indicate eosinophils. (c) Percentage eosinophils in bone marrow, and total eosinophils in blood (d) and bronchoalveolar lavage (BAL) (e) from IL‐33‐ or PBS‐treated mice. Eotaxin‐1/CCL11 (f) and eotaxin‐2/CCL24 (g) protein levels in serum, and eotaxin‐1/CCL11 (h) and eotaxin‐2/CCL24 (i) protein levels in BAL from IL‐33‐ or PBS‐treated mice. (j) Percentage eosinophils in BAL from mice treated with IL‐33 in combination with anti‐eotaxin‐2/CCL24 (αEot‐2) or isotype control. Data are shown as means (±SEM in f–i) (n = 4 to n = 14 per group). *P < 0·05, **P < 0·01, and ***P < 0·001. [Colour figure can be viewed at wileyonlinelibrary.com]