Figure 3.
Inhibition of hypothermia‐induced increase in AQP4 cell surface localization in primary human astrocytes. Data are mean fold changes in expression (±SEM) of cells incubated at 32 °C for 4 h (Hypothermia) or 85 mOsm/kg H2O (Hypotonicity) compared with normothermic (Control) astrocytes with or without the indicated inhibitors. The calmodulin inhibitor is 127 μm TFP; the TRPV4 inhibitor is 4.8 μm HC‐067047; the intracellular calcium chelator is 5 μm EGTA AM. Kruskal–Wallis with Conover–Inman post hoc analysis tests were used to identify significant differences between samples (n = 4). ***Represents statistical significance (***P < 0.001) using statsdirect 3 software.