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. 2017 Nov 7;123(6):1584–1596. doi: 10.1111/jam.13590

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© 2017 The Authors. Journal of Applied Microbiology published by John Wiley & Sons Ltd on behalf of The Society for Applied Microbiology.

This is an open access article under the terms of the Creative Commons Attribution License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited.

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16S rRNA gene sequencing sensitivity and accuracy. Expected (grey bars) versus observed (black bars) ratios for binary mixtures of DNA and live bacteria ranging from 0·1 to 99·9% for each organism are graphed. Error ratio (striped bars), total count of species identified that were not added to the mixture divided by total number of reads. ■ Observed ratio; Expected ratio; Error ratio.

Inline graphic — 16S rRNA gene sequencing sensitivity and accuracy. Expected (grey bars) versus observed (black bars) ratios for binary mixtures of DNA and live bacteria ranging from 0·1 to 99·9% for each organism are graphed. Error ratio (striped bars), total count of species identified that were not added to the mixture divided by total number of reads. ■ Observed ratio; Expected ratio; Error ratio.