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. 2017 Oct 23;115(1):192–205. doi: 10.1002/bit.26454

Figure 1.

Figure 1

Engineered in vitro GDP‐mannose pathway with enzymes from different microorganisms expressed in E. coli: Glucokinase (His6‐Glk), phosphomannomutase (ManB‐His6) and mannose‐1‐phosphateguanyltransferase (ManC), inorganic pyrophosphatase (PmPpA‐His6), 1‐domain polyphosphate kinase 2 (His6‐1D‐Ppk2) in vitro coupled to β‐1,4‐mannosyltransferase (Alg1ΔTM) for the production of phytanyl‐PP‐(GlcNAc)2‐Man1 with GDP‐mannose regeneration