Official websites use .gov
A
.gov website belongs to an official
government organization in the United States.
Secure .gov websites use HTTPS
A lock (
) or https:// means you've safely
connected to the .gov website. Share sensitive
information only on official, secure websites.
Engineered in vitro GDP‐mannose pathway with enzymes from different microorganisms expressed in E. coli: Glucokinase (His6‐Glk), phosphomannomutase (ManB‐His6) and mannose‐1‐phosphateguanyltransferase (ManC), inorganic pyrophosphatase (PmPpA‐His6), 1‐domain polyphosphate kinase 2 (His6‐1D‐Ppk2) in vitro coupled to β‐1,4‐mannosyltransferase (Alg1ΔTM) for the production of phytanyl‐PP‐(GlcNAc)2‐Man1 with GDP‐mannose regeneration
