Figure 4.

(a) Irreversible man6P synthesis by His₆‐Glk (1 mg/ml) from ATP (1.7 mM) using different mannose concentrations. The lines represent the model (see Table 1). (b) Reverse reaction of the ManB‐His₆/ManC (0.171 mg/ml) complex: GDP‐mannose and pyrophosphate are converted to GTP and man6P catalyzed by ManB and ManC. Subsequently, the synthesis of the GDP‐man is largely dependent on the conversion of pyrophosphate to phosphate. The fits depicted are fits to the entire data set of His₆‐Glk and ManB‐His₆/ManC, respectively, catalyzed reactions