Figure 4. Correlations of ApoJ contents in HDL and LDL fraction and risk factor for insulin resistance in human subjects.
(a) Serum lipoproteins (HDL and LDL) in iodixanol gradients were separated by Sudan Block containing agarose gel. (b) ApoJ proteins in HDL and LDL fraction were separated by SDS/PAGE and transferred to nitrocellulose membranes. Each lane in the blot represents a different subject. The immunoblots shown are representative of three blots (H: HDL, L: LDL). Bars show densitometric quantitation of ApoJ from HDL and LDL fractions. Results are mean ± SEM for 9 subjects per group. *P<0.0001 vs. ApoJ from LDL.
(c–f) Relationship of ApoJ bound to HDL or LDL with HOMA-IR and fasting insulin levels.