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. Author manuscript; available in PMC: 2018 Oct 18.
Published in final edited form as: Chembiochem. 2017 Sep 1;18(20):2028–2032. doi: 10.1002/cbic.201700137

Figure 1. N-ethyl maleimide reacts with NEM but not IAM.

Figure 1

Reactions were carried out in 10:1 (electrophile : nucleophile) in degassed 6 M urea / citrate-phosphate buffer (pH 4.5) for 2 hours and assayed by high resolution LC-MS. Similar yields were achieved at pH 7.4 in phosphate buffer. Values represent conversion efficiency of the reaction mixture measured by LC-MS. Supporting LC-MS extracted ion chromatographs are reported in Figure S2.