Fig. 6.

—Recently acquired genes from the cloud genome of B. subtilis have homologous copies within closely related groups. Taxonomic characterization of best BLAST hits of each protein from the cloud genome of B. subtilis indicates that 72.3% of these genes have a close homologue within the genus Bacillus and an additional 9.1% within the phylum Firmicutes. Eighty-five genes (2.0%) have best BLAST hits with species from other phyla, such as Proteobacteria and Bacteroidetes, which is interpreted as evidence of long-distance LGT. POE (16.6%) represents putative orphan (or error) genes; these are B. subtilis genes with no homologues in other species represented in the NCBI nr database. These results were obtained after a BLASTP search for each protein from the cloud genome against the NCBI nr database excluding B. subtilis entries using an e-value threshold of 10⁻⁵.