Fig. 5.

Functional analyses of the Zymoseptoria tritici pangenome. a Lengths of the encoded proteins by core, accessory, and singleton genes of the pangenome (different letters are P < 0.0001, Kruskal–Wallis test). b Percentage of proteins with a conserved protein domain (different letters are P < 0.0001, Fisher exact test). c, d Percentage of genes encoding secreted proteins and candidate effectors, respectively (different letters are P < 0.0001, Fisher exact test). e Venn diagram of shared and unique effector candidates in the pangenome of isolates 1A5, 1E4, 3D1, 3D7, and IPO323 (reference genome). f Median expression levels of pangenome genes (different letters are P < 0.0001, Kruskal–Wallis test)