Effects of CCE on cell viability and wound healing assay in human lung cancer A549 and H1299 cells. (A) A549 (B) H1299 (C) MRC-5 and (D) WI-38 cells were treated with different concentrations (0, 20, 40, and 60 μg/mL) of CCE for 24 and 48 h prior to MTT assay for cell viability. The wound healing assay was conducted as described in the Materials and Methods section after (E) A549 and (F) H1299 cells were treated with different concentrations (0, 20, 40, and 60 μg/mL) of CCE for 24 and 48 h. micrograph: 40×. Scale bar, 50 μm. Results were statistically evaluated using one-way ANOVA with post hoc Dunnett's test (**, P<0.01; ***, P<0.001). Results from three repeated and separate experiments were similar.