Table 2. Systems subjected to HRF analysis (in vitro reconstituted nucleosomes or free DNA).
Identifier | DNA | Histone content |
---|---|---|
601TA-NUC | 601TA | H4, H2A, H2B − canonical histones + Cse4p (centromeric variant of H3), all from S. cerevisiae |
CEN3-NUC_1b | CEN3c | |
CEN3-NUC_2 | ||
CEN3-NUC_3 | ||
CEN3-free | CEN3 | free DNA |
bThree independent experiments.
cCentromere DNA sequence of Chromosome III in S.cerevisiae, 136 bp long.