Fig. 2.

Inhibition or depletion of PARP or PARG increases sensitivity to ionising radiation. (A) Survival fraction of MCF-7 cells untreated (DMSO), treated with PARG inhibitor (PDD00017273), PARP inhibitor (olaparib), or both for 16 h prior to and during recovery from ionising radiation (IR). (B) Survival fraction following IR of siRNA transfected cells as indicated. Survival was measured by clonogenic survival assay. Mean and standard deviation of three independent repeats are shown. Statistical significance calculated by Student’s T-test, cf. to DMSO or scrambled siRNA control, where *** represents p < 0.001. (C) Protein expression of siRNA transfected cells 48 h post-transfection.