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Nanoassembly-mediated delivery of functional Granzyme A-E10 (GzmA-E10) effectively killed HeLa cells. Confocal microscopy images showing FITC-GzmA-E10 or GFP-E10 delivered cells along with phosphatidylserine staining. a) 3, and 24 h after GzmA-E10 delivery. b) 24 h after GFP-E10 delivery. Note that 24 h after GzmA-E10 delivery cells died, which also showed phosphatidylserine staining, confirming the GzmA-E10 mediated cell death. Scale bar= 20 μm.
