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. Author manuscript; available in PMC: 2018 Feb 28.
Published in final edited form as: Nat Struct Mol Biol. 2017 Aug 28;24(10):816–824. doi: 10.1038/nsmb.3455

Fig. 2. Function of NONO-PSF and NEAT1 analyzed with the pri-miRNA processing reporter.

Fig. 2

(a) Top: The pri-miRNA processing reporter containing pri-miR-17–92a cloned into the 3′UTR of the Renilla luciferase in psiCHECK2 vector. (b) Relative luciferase activities of the pri-miRNA processing reporter in response to knockdown of individual RBPs as indicated or NEAT1_V2. (c) Relative luciferase activities of the pri-miRNA processing reporter in response to overexpression of siRNA-resistant PSF (left) or NONO (right). Bar graphs in b and c are presented as mean ± SEM (n=3, cell culture). *P < 0.05; **P < 0.01; ***P < 0.001; NS, not significant, determined by two-tailed Student’s t test. Data source for the bar graphs are reported in Source Data for Figure 2. Uncropped images of Western blots in c are shown in Supplementary Data Set 1.