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. Author manuscript; available in PMC: 2018 Feb 28.
Published in final edited form as: Nat Struct Mol Biol. 2017 Aug 28;24(10):816–824. doi: 10.1038/nsmb.3455

Fig. 4. NEAT1 bridges NONO-PSF and the Microprocessor.

Fig. 4

(a) Co-IP of endogenous NONO with PSPC1 and the Microprocessor DROSHA/DGCR8. (b) RNA-dependent interactions between NONO-PSF and the Microprocessor. (c) RT-PCR analysis of NEAT1_V1, NEAT1_V2, and MALAT1 in NONO and DGCR8 immunoprecipitants. (d) Knockdown efficiency of NEAT1_V2 and MALAT1, quantified by RT-qPCR and normalized against GAPDH mRNA. (e, f) Western blotting analysis of NONO and the Microprocessor interactions in response to knockdown of NEAT1_V2 (e) or MALAT1 (f). * indicates IgG heavy chain. Uncropped images of western blots in a,b,c,e, and f are shown in Supplementary Data Set 1. Bar graphs in d are presented as mean ± SEM (n=3, technical replicates). ***P < 0.001, determined by two-tailed Student’s t test. Data source for the bar graphs are reported in Source Data for Figure 4.