Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2017 Oct 31;15(1):453–458. doi: 10.3892/ol.2017.7305

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

Copyright © 2018, Spandidos Publications

PMC Copyright notice

Figure 3. — miR-100 regulates the expression of CXCR7 in vitro. (A) RT-qPCR analysis of the expression of miR-100 in gastric cancer cell line (BGC823) after miR-100 mimic, inhibitor, and negative control miRNA transfection. (B) MTT assay to determine the level of cell proliferation in gastric cancer cell line (BGC823) after miR-100 mimic, inhibitor, and negative control miRNA transfection. (C) Putative miR-100-binding sequence within the 3′-UTR of CXCR7 mRNA. (D) Luciferase gene reporter gene assays were performed to verify the relationship between miR-100 and CXCR7. (E) RT-qPCR and (F) Western blot analysis of the expression of CXCR7 in gastric cancer cell line (BGC823) and normal gastric epithelial cell line (GES-1). (G) RT-qPCR analysis of the expression of CXCR7 in gastric cancer cell line (BGC823) after miR-100 mimic, inhibitor, and negative control miRNA transfection. (H) RT-qPCR analysis of the expression of CXCR7 in gastric cancer cell line (BGC823) after CXCR7-sepcific siRNA and negative control siRNA transfection. (I) MTT assay to determine the level of cell proliferation in gastric cancer cell line (BGC823) after CXCR7-sepcific siRNA and negative control siRNA transfection (NS: Not significance, *P<0.05, **P<0.01, ***P<0.001). miR-100, microRNA-100; CXCR7, Chemokine (CXC motif) receptor 7; siRNA, small-interfering RNA; OD₄₉₀, optical density at 490 nm.