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. 2017 Oct 31;15(1):175–182. doi: 10.3892/ol.2017.7295

Figure 3.

Figure 3.

Effects of S100A11 overexpression on cell proliferation, cell apoptosis and cell cycle distribution in human pancreatic cancer PANC-1 cells. PANC-1 cells were transfected with 2.5 µg pcDNA3.1-S100A11 and pcDNA3.1 emptor vector using Lipofectamine 2000 for 48 h. (A) S100A11 mRNA expression was determined by semi-quantitative reverse transcription-polymerase chain reaction analysis. β-actin was used as a control. (B) S100A11 protein expression was determined by western blot analysis. β-actin was used as a control. (C) Cell proliferation was determined using a cell counting kit-8 assay. (D) Cell apoptosis was determined by flow cytometry analysis using Annexin V/PI staining. (E) Cell cycle distribution was determined by flow cytometry analysis using PI staining. Data were presented as the mean ± standard error of mean following three independent experiments. *P<0.05 vs. empty vector. PI, propidium iodide.