Figure 4.

Effects of S100A11 overexpression on AKT mRNA expression, and p-AKT and total AKT protein expression in PANC-1 cells. (A) AKT mRNA expression was determined by semi-quantitative reverse transcription-polymerase chain reaction analysis. β-actin was used as a control. (B) Total AKT and p-AKT protein expression was determined by western blot analysis. β-actin was used as a control. Data were presented as the mean ± standard error of mean following three independent experiments. *P<0.05 vs. empty vector. p, phosphorylated; AKT, AKT serine/threonine kinase.