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. 2017 Oct 31;15(1):175–182. doi: 10.3892/ol.2017.7295

Figure 5.

Figure 5.

Effects of PI3K inhibitor LY294002 on cell proliferation, apoptosis, cell cycle distribution, mRNA expression of S100A11 and AKT, and protein expression of S100A11, p-AKT and total AKT in PANC-1 cells. PANC-1 cells were treated with 50 µmol/l LY294002 for 48 h. (A) mRNA expression of S100A11 was determined by semi-quantitative reverse transcription-polymerase chain reaction analysis. β-actin was used as an internal control. (B) Protein expression of S100A11, p-AKT and total AKT were determined by western blot analysis. β-actin was used as an internal control. (C) Cell proliferation was determined using a cell counting kit-8 assay. (D) Cell apoptosis was determined by flow cytometry analysis using Annexin V/PI staining. (E) Cell cycle distribution was determined by flow cytometry analysis using PI staining. Data were presented as the mean ± standard error of mean following three independent experiments. *P<0.05 vs. control. p, phosphorylated; AKT, AKT serine/threonine kinase; PI, propidium iodide.