Supplementary Figure 1.

EGFR expression and phosphorylation in tumor and stromal cells of patients with CRF. (A) Representative images used for IHC scoring of EGFR expression (−, +, ++, +++) in tumor and stromal cells (upper panels). Intensities of EGFR determined by IF (lower panels) were comparable with IHC. (B) Distribution of tumors according to the percentage of CD68⁺/EGFR⁺ (n = 118), CD11b⁺/EGFR⁺ (n = 108), and CD11b⁺/pEGFR⁺ (n = 113) cells in the total CD68⁺ or CD11b⁺ stromal population, respectively, determined by IF double staining shown in (C) and Figure 1F. (C) IF double staining on CRC samples shows presence of CD11b⁺/pEGFR⁺ cells in the stroma. Scale bars 50 μm. (D) Patients were divided into 2 groups: low (0 to <1%) and high (≥1%), according to percentage of CD11b⁺/pEGFR⁺ cells in the total CD11b⁺ stromal population (n = 113), determined by IF shown in (C).