Supplementary Figure 2.

EGFR expression and intestinal development. (A) EGFR IHC staining on DSS-treated colons of indicated mice, black arrowheads depict IECs, white arrowheads depict LP cells. (B) PCR and (C) Southern blot analysis of genomic DNA from purified colonocytes showing successful Cre-mediated recombination of the floxed Egfr allele in Egfr^ΔIEC mice. (D) Western blot confirming absence of EGFR protein in purified colonocytes from Egfr^ΔIEC mice. (E) PCR analysis of purified bone marrow–derived macrophages showing successful Cre-mediated recombination of the floxed Egfr allele in Egfr^ΔMYL mice. (F) Western blot confirming loss of EGFR protein in bone marrow–derived macrophages from Egfr^ΔMYL mice. (G) Quantitative reverse transcriptase PCR analysis of EGFR mRNA expression of bone marrow–derived macrophages showing absence of EGFR in Egfr^ΔMYL mice. mRNA expression levels were normalized to TBP (Egfr^f/f n = 2, Egfr^ΔMYL n = 5). (H) Hematoxylin-eosin (H&E) and periodic acid-Schiff (PAS) staining on colon sections, arrowheads depict goblet cells. Giemsa staining on small intestine, arrowheads point to refractive eosinophilic granules of paneth cells. IHC staining for lysozyme and synaptophysin, arrowheads demonstrate presence of paneth cells and enteroendocrine cells, respectively. Scale bars 50 μm.