Supplementary Figure 3.

EGFR signaling in myeloid cells in the AOM/DSS-dependent model of CAC. (A) Scheme of AOM/DSS-administration to induce CAC; 7.5 mg/kg AOM was injected IP; DSS was given in drinking water (black areas) followed by regular water (white areas). (B) IF double staining for CD64 and pEGFR. Arrowheads depict presence of CD64⁺/pEGFR⁺ cells. (C) Grading of neoplastic lesions. Percentage of low-grade (LG) and high-grade (HG) adenomas as well as adenocarcinomas in indicated genotypes (n ≥4). (D) Hematoxylin-eosin stainings of different lesion grades as indicated. (E) Histological assessment of inflammation scores on colon sections of AOM/DSS-treated mice (n ≥4). (F) BrdU IF and cleaved Caspase-3 IHC stainings, arrowheads depict BrdU⁺ and cleaved Caspase-3⁺ nuclei in tumor cells, respectively. Scale bars 50 μm.