Supplementary Figure 4.

EGFR signaling in myeloid cells promotes Apc^Min-driven CRC development via STAT3 activation in tumor cells. (A) IHC staining for pSTAT3 and survivin in the Apc^Min/+ model, arrowheads depict pSTAT3⁺ and survivin⁺ nuclei in tumor cells, respectively. Scale bars 50 μm. (B) pSTAT3 index was defined by dividing the number of pSTAT3⁺ tumor cells by the tumor area in Apc^Min/+ mice (13–25 tumors from 3–4 different mice were counted). (C) Survivin index was defined by dividing the number of survivin⁺ tumor cells by area of tumors in Apc^Min/+ mice (9–30 tumors from 3–5 different mice were counted). Data are mean ± SEM. ***P < .001, t test. #P < .05, ###P < .001, 1-way analysis of variance with Dunn’s posttest.