Figure 6.

Forced Rst2 binding to UAS2 causes unregulated fbp1 transcription. (A) Schematic representation of Rst2 tethering using Gal4-GBD. Three copies of Gal4-binding sequences (BS) were inserted at the upstream adjacent region of UAS2. Gal4-GBD was fused to the C-terminus of Rst2. The resultant fusion protein is tethered at Gal4-BS. (B) Gal4-GBD fusion mediated tethering of Rst2 to UAS2 causes fbp1 activation even in the presence of glucose. fbp1 transcriptional activation was examined with northern blot analysis in Rst2-Gal4-GBD cells. (C) Schematic representation of the translocation of region-b to the upstream adjacent region of UAS2. (D, E) Translocation of novel Rst2 binding site, region-b to upstream adjacent of UAS2 bypasses requirement of higher order structure formation for fbp1 induction. Transcripts of fbp1 were examined with northern blot analysis in indicated cells.