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. 2017 Jul 26;45(16):9583–9594. doi: 10.1093/nar/gkx634

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© The Author(s) 2017. Published by Oxford University Press on behalf of Nucleic Acids Research.

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by-nc/4.0/), which permits non-commercial re-use, distribution, and reproduction in any medium, provided the original work is properly cited. For commercial re-use, please contact journals.permissions@oup.com

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Figure 7. — UbaLAI reaction mechanism. (A) UbaLAI–DNA association. The first DNA site is bound via the non-catalytic UbaLAI-N domain. This triggers binding and cleavage of the second DNA site by the catalytic UbaLAI-C domain. (B) The proposed UbaLAI reaction mechanism (‘sequential nicking via tethered activation’) on 2-site DNA, involving the ‘swiveling’ catalytic UbaLAI-C domain and the UbaLAI-N domain that tethers the catalytic domain to the multi-site DNA. (C) Proposed mechanism for the UbaLAI-2mut reaction on the 2-site DNA. The complex dissociates before UbaLAI-C can re-bind in the opposite orientation and complete the reaction. (D) Wt UbaLAI reaction on 1-site DNA. The in trans synaptic complex dissociates after nicking the first DNA strand.