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. 2017 Jul 10;45(16):9654–9678. doi: 10.1093/nar/gkx606

Figure 6.

Figure 6.

(A) Western blots of various RNA binding proteins in two independent preparations of nuclear extracts (Prep 1 and Prep 2) prepared from C33A2 cells treated with DMSO (D) or 100 uM Akt kinase inhibitor GDC0068 (G) for 3 h. Western blots of indicated proteins pulled down with biotinylated ssDNA oligos (B) or RNA oligos (C) representing sequences at the HPV16 3′-splice site SA3358 in the E4-coding exon. (D) Western blots of indicated proteins pulled down with biotinylated ssDNA oligos representing sequences at the HPV16 late 5′-splice site SD3632 in the E4-coding exon. (E) Western blots of indicated proteins pulled down with biotinylated ssRNA oligos spanning HPV16 5′-splice sites SD880 (BRnSD862) and SD3632 (BRnSD3632). (F) Western blots of hnRNP L, hnRNP A1, U2AF65 or U2AF35 pulled down from nuclear extracts using ssDNA oligos at HPV16 late splice site SA5639. For all pull downs in the figure, nuclear extracts prepared from C33A2 cells treated with DMSO (D) or Akt kinase inhibitor GDC0068 (G) were used.