Figure 6.
Effect of PolδD316A;E318A on susceptibility to killing by MNNG. (A and B) HeLa cells were transfected and seeded as described in Materials and Methods. After one day, cells were treated with 10 μM O6-Benzylguanine and MNNG at the concentration indicated. Colonies were counted after 8 days after crystal violet staining. The percentage of survival for each drug concentration was calculated as number of colonies after MNNG treatment / number of colonies without MNNG treatment. The asterisk above the error bar indicates the P value between group siEXO1+Polδ and group siEXO1+PolδD316A;E318A. (C and D) Cells were seeded into T75 flasks one day after transfection. After 24 hours, cells were treated with 10 μM O6-Benzylguanine and 0.2 μM MNNG and incubated for another two days before subject to FACS analysis. The data represent the mean ± SD of three independent experiments. *P ≤ 0.05.