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. 2017 Aug 1;45(16):9797–9808. doi: 10.1093/nar/gkx698

Figure 4.

Figure 4.

Characterization of the orthogonality of two designer RNA hybridization networks (trigR11 and trigR2) in bacterial cell populations. (A) Scheme of the system trigR11 (with cognate sRNAs and 5′ UTR). (B) Scheme of a crossed system with non-cognate elements, where the sRNAs correspond to system trigR2 and the 5′ UTR corresponds to system trigR11. Promoters PLlacO1 and PLtetO1 control the expression of the two sRNAs (SR and SRR), which can be tuned with external inducers IPTG and aTc, whereas the mRNA (SRRR:sfGFP) is constitutively expressed from promoter J23119. (C) Fluorescence results (arbitrary units, AU) from the systems shown in (A) and (B). Error bars represent standard deviations over three biological replicates. (D) Scheme of the system trigR2 (with cognate sRNAs and 5′ UTR). (E) Scheme of a crossed system with non-cognate elements, where the sRNAs correspond to system trigR11 and the 5′ UTR corresponds to system trigR2. (F) Fluorescence results (arbitrary units, AU) from the systems shown in (D) and (E). Three biological replicates. In both cases, the asterisk denotes P < 0.05, one-tailed Welch t-test, comparing the fluorescence level for the cognate pair with respect to the level for the non-cognate pair.