Figure 6.

Molecular characterization of designer RNA hybridization networks in vitro. (A, C) Structures of the species implementing the systems trigR2 and trigR31. The toehold for the interaction between the two sRNAs is shown in light blue. The toehold for the interaction between the heterodimer (sRNA complex) and the 5′ UTR is shown in red. In the 5′ UTR SRRR2 the RBS works as the downstream control element, while in SRRR31 the start codon AUG does (both shown in yellow). The transcription terminator T500 was used in SRR2 and SRR31, while the terminator TrrnC was used in SR2 and SR31. (B, D) Electrophoretic analysis showing the hierarchical interaction between sRNAs. The formation of the heterodimer and heterotrimer is shown in lanes 6 and 7, respectively.