Fig 1. Schematic diagram of Ywp1 and its encoding segments that were included in DNA vaccines.

Four segments of YWP1, encoding all 533 amino acids of Ywp1 or just amino acids 21–116, 51–197 or 105–161, were included in DNA vaccines for the elicitation of specific antibodies in mice [22]. Features of Ywp1 are noted here for their locations relative to these segments: The cleaved signal peptide (aa 1–21), the sole site for N-glycan addition (N115), the tribasic and dibasic propeptide cleavage sites (RRR and KR), the sites for insertion of a fluorescent tag (Gfp) and an epitope tag (HA) (described in subsequent sections), and the site of GPI anchorage (ω511), a remnant of which mediates covalent linkage of Ywp1 to the cell wall matrix.