Skip to main content
. 2018 Jan 12;13(1):e0191194. doi: 10.1371/journal.pone.0191194

Fig 8. Micrographs of strain YGY and derivatives.

Fig 8

DIC images (A-E) and corresponding Gfp fluorescence images (A’-E’). Except as indicated, yeast cells were grown to stationary phase in phosphate-limited BMM13 and dispersed in pH 8 buffer for photomicrography. (A-A”) Overlay shows coincident localization of Gfp fluorescence and the yeast cell wall. (B,B’) Strain YGY; (C,C’) strain YGY derivative with its wild type YWP1 allele knocked out; (D,D’) strain YGY with its YWP1-GFP-YWP1 allele knocked out (and 5× greater photographic exposure of fluorescence); (E,E’) same strain as in panels C and C’ but grown in unbuffered MM13 (4× greater photographic exposure). Actual width of each image (μm): A: 24; B-E: 76.