Fig 10. Effects of phosphate limitation on strain YGY.

(A,A’) Yeast cells were grown on the surface of rich (phosphate-replete YPD) nutrient agarose; fluorescent Ywp1-Gfp-Ywp1 was often more abundant in the walls of nascent daughters than in their mother cells. When starved of phosphate by growth to stationary phase in low phosphate BMM13, yeast cells accumulated abundant Ywp1-Gfp-Ywp1 in their walls; when placed in fresh medium, these cells budded daughters with less wall fluorescence (B,B’). A range of Ywp1-Gfp-Ywp1 accumulations arose within the population as phosphate was depleted from growing cultures; phosphate depletion also resulted in reduction in the quantity of phosphodiester-linked mannotriose in the cell wall, as shown by immunolabeling with a monoclonal antibody (G11.1) specific for the mannotriose and a fluorescent red (eFluor660) secondary antibody (C-C”‘). Note the frequent complementarity of signal intensities. Note also that the red signal sometimes appears exterior to the green, possibly resulting from a combination of the mannotriose extending farther from the wall and the double-antibody bridge. Actual width of each image (μm): A: 76; B: 76; C: 54.