Skip to main content
. 2018 Jan 2;14(1):e1006811. doi: 10.1371/journal.ppat.1006811

Table 1. Indole-3-acetic acid (IAA) levels in culture.

Strain Supplementa IAA ng/ml
24hrb
IAA ng/ml
48hrb
DC3000 N/A 28.9 ± 4.6 30.6 ± 3.5
DC3000 Trp 2520 ± 245 2760 ± 259
DC3000 IAAld 3700 ± 189 11700 ± 657
DC3000 IAM 144 ± 18 100 ± 11
DC3000 IAN 190c ± 7 301c ± 10
DC3000 IPyA 8820d ± 331 14100d ± 285
DC3000 TAM 107 ± 20 147 ± 10

aDC3000 cultures grown in Hoitken-Sinden media with 10 mM citrate (HSC) and 0.25 mM of the indicated supplement.

bHSC media supplemented with Trp, IAAld, IAM, or TAM accumulated no detectable levels of IAA in the absence of bacteria after 24 or 48 hrs of incubation. Values are average ± SEM (n = 3).

cHSC media containing IAN but lacking DC3000 accumulated 111 ng/ml and 124 ng/ml of IAA at 24 and 48 hrs of incubation, respectively.

dHSC media supplemented with IPyA but lacking DC3000 accumulated 17,000 ng/ml and 16,300 ng/ml of IAA at 24 hrs and 48 hrs of incubation, respectively. Similar results were obtained in two additional independent experiments.