Table 1. Indole-3-acetic acid (IAA) levels in culture.
Strain | Supplementa | IAA ng/ml 24hrb |
IAA ng/ml 48hrb |
---|---|---|---|
DC3000 | N/A | 28.9 ± 4.6 | 30.6 ± 3.5 |
DC3000 | Trp | 2520 ± 245 | 2760 ± 259 |
DC3000 | IAAld | 3700 ± 189 | 11700 ± 657 |
DC3000 | IAM | 144 ± 18 | 100 ± 11 |
DC3000 | IAN | 190c ± 7 | 301c ± 10 |
DC3000 | IPyA | 8820d ± 331 | 14100d ± 285 |
DC3000 | TAM | 107 ± 20 | 147 ± 10 |
aDC3000 cultures grown in Hoitken-Sinden media with 10 mM citrate (HSC) and 0.25 mM of the indicated supplement.
bHSC media supplemented with Trp, IAAld, IAM, or TAM accumulated no detectable levels of IAA in the absence of bacteria after 24 or 48 hrs of incubation. Values are average ± SEM (n = 3).
cHSC media containing IAN but lacking DC3000 accumulated 111 ng/ml and 124 ng/ml of IAA at 24 and 48 hrs of incubation, respectively.
dHSC media supplemented with IPyA but lacking DC3000 accumulated 17,000 ng/ml and 16,300 ng/ml of IAA at 24 hrs and 48 hrs of incubation, respectively. Similar results were obtained in two additional independent experiments.