Figure 2.
(A) Inhibition assays comparing the effect of EcN WT and EcN pJPMcH47 against lawns of pathogenic S. Typhimurium (left) and E. coli DH5α (right) on minimal media, supplemented with 22 mM L-rhamnose and 0.2 mM 2,2′-dipyridyl (see Methods). (B) Measurement of inhibition area in the S. Typhimurium lawn as a function of L-rhamnose concentration on LB agar plates supplemented with variable L-rhamnose (0.025–10 mM) and 0.2 mM 2,2′-dipyridyl. 2,2′-Dipyridyl addition was necessary for maximum S. Typhimurium inhibition by MccH47 (see Methods and Figure S2).