Figure 4.
Tetrathionate-induced inhibition by EcN pttrMcH47 MccH47 production. (A) Inhibition assays comparing the ability of three separate clones of EcN pttrMcH47 and EcN WT to inhibit growth of S. Typhimurium in the absence (left) and presence (right) of 1 mM potassium tetrathionate (a,b indicate duplicate stabs).·(B) Results from in vitro ecological competition experiments, showing the proportion of S. Typhimurium WT (left) or S. Typhimurium pBR322 (right) over time. (C) S. Typhimurium fitness estimation obtained from competition experiments (see ref 21). Supplementation of 1 mM potassium tetrathionate leads to production of MccH47 and a statistically significant reduction (p < 0.05) in S. Typhimurium fitness.