Table 1.

Strains, Plasmids, and Oligonucleotides

	relevant characteristics/sequence (5′–3′)	source or reference
strains
Escherichia coli Nissle 1917		27
Salmonella Typhimurium	Salmonella enterica subsp. enterica serovar Typhimurium ATCC 29630	ATCC
Escherichia coli NEB10β	Δ(ara-leu) 7697 araD139 fhuA ΔlacX74 galK16 galE15 e14-ϕ80dlacZΔM15 recA1 relA1 endA1 nupG rpsL (StrR) rph spoT1 Δ(mrr-hsdRMS-mcrBC)	New England Biolabs
Escherichia coli DH5α	F– Φ80lacZΔM15 Δ(lacZYA-argF) U169 recA1 endA1 hsdR17 (rK−, mK+) phoA supE44 λ– thi-1 gyrA96 relA1	New England Biolabs
plasmids
pBR322	pMB1, ApR, TcR	New England Biolabs
pLJV3	pUC57, CFP, mchA, rhaPBAD	this study; synthesized by General Biosystems
pJPMcH47	rhaPBAD, mchAXIBCDEF	this study
pttrMcH47	ttrRSBCA, mchAXIBCDEF	this study
pEX2000	pBR322, mchAXIBCDEF	29
oligonucleotides
pBR322FWD	GGATTATTTCTATTTAAAATGAGGCCCTTTCGTCTTCAAGAATTCT
pBR322REV	GAGATAGCGGTAGCTAACTAGACGTCAGGTGGCACTTTTCG
pLJV3FWD	TTACCAGACCTCACCCAGACTTCATTACGACCAGTCTAAAAAGCGCCTG
pLJV3REV	GAAAAGTGCCACCTGACGTCTAGTTAGCTACCGCTATCTCCAACGTGC
pEX2000FWD	TTTAGACTGGTCGTAATGAAGTCTGGGTGAGGTCTGGTAAGA
pEX2000REV	CTTGAAGACGAAAGGGCCTCATTTTAAATAGAAATAATCCTGTCAACAGTTCTCAACG
pJPMcH47FWD	AAAAATCGAGCGTATATAACGTCTGGGTGAGGTCTGGTAAGA
pJPMcH47REV	TCTGTTGGTTTGATCTGGCGGGATGTGACGATCGTTGACAGC
SentFWD	TTACCAGACCTCACCCAGACGTTATATACGCTCGATTTTTGCCGGC
SentREV	TGTCAACGATCGTCACATCCCGCCAGATCAAACCAACAGAA