Table 2.
Relative fitness of WNV isolates after in vivo competition in HOFIs and Cx. tarsalis.
| Isolate | HOFI | Cx. tarsalis | |||
|---|---|---|---|---|---|
|
| |||||
| RNA 3 dpi | RNA 6 dpi | RNA body | Infection | Transmission | |
| COAV-07-1573 | wildtype** a | neutral (ns) | wildtype*** | wildtype (1.41) b | wildtype (3.16) |
| COAV-08-1091 | neutral (ns) | COAV997-5nt | COAV997-5nt * | wildtype (1.05) | COAV997-5nt (0.61) |
| COAV-08-1333 | wildtype * | neutral (ns) | wildtype *** | wildtype (1.24) | wildtype (1.40) |
| COAV-08-1722 | wildtype *** | neutral (ns) | wildtype *** | wildtype (1.17) | wildtype (1.41) |
| GRLA-07-5894 | wildtype ** | neutral (ns) | neutral (ns) | wildtype (1.33) | neutral (1.00) |
| GRLA-08-277 | wildtype ** | wildtype ** | wildtype ** | wildtype (1.23) | wildtype (1.83) |
| GRLA-08-5546 | wildtype ** | wildtype * | neutral (ns) | wildtype (1.62) | wildtype (1.69) |
| GRLA-08-6006 | wildtype ** | wildtype * | neutral (ns) | wildtype (1.30) | wildtype (1.14) |
| KERN-07-477 | wildtype ** | wildtype | wildtype *** | wildtype (1.12) | wildtype (1.40) |
| KERN-08-136 | wildtype ** | neutral (ns) | wildtype *** | wildtype (1.33) | wildtype (1.12) |
| KERN-08-360 | wildtype ** | wildtype | neutral (ns) | neutral (1.00) | COAV997-5nt (0.57) |
| KERN-08-377 | wildtype ** | wildtype | wildtype *** | wildtype (1.83) | wildtype (2.36) |
| SAYO-07-6588 | wildtype ** | neutral (ns) | neutral (ns) | wildtype (1.61) | COAV997-5nt (0.77) |
| SAYO-08-2604 | wildtype ** | wildtype | n/a | wildtype (1.50) | wildtype (3.00) |
| SAYO-08-4541 | wildtype * | neutral (ns) | wildtype *** | wildtype (1.09) | wildtype (2.00) |
| SAYO-08-6803 | wildtype ** | neutral (ns) | neutral (ns) | wildtype (2.50) | wildtype (2.25) |
Statistical significance inferred from RNA copy differences in HOFI sera and mosquito bodies is indicated either as significant (*, P<0.05), very significant (**, P<0.01), extremely significant (***, P<0.001) or not significant (ns, P>0.05).
An additional measure of fitness was derived from mosquito infection and transmission rates, by dividing the number of bodies and expectorants positive for wildtype-RNA by those positive for COAV997-5nt, respectively, and fitness determined as either neutral (1.00), wildtype (>1.00) or COAV997-5nt (<1.00).
Blue and red color highlight either the wildtype isolate or the marked founding California strain, respectively, as winners of the fitness competition, while green color indicates neutral fitness.