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. Author manuscript; available in PMC: 2019 Jan 15.
Published in final edited form as: Neuroscience. 2017 Nov 20;369:248–260. doi: 10.1016/j.neuroscience.2017.11.018

Figure 4. Regulation of Ppp3r1 mRNA expression by ING1 is mediated by the Piwi pathway.

Figure 4

Piwil1 (A) and Piwil2 (B) mRNA is detected in primary cortical neurons at baseline; mRNA of both Piwil1 and Piwil2 significantly increases following 3 hours of KCl stimulation (n=4–6, Student’s t-test, *p<0.05, **p <0.01). Expression of Piwil1 (C) and Piwil2 (D) is significantly reduced in primary cortical neurons 4 days after treatment with the respective shRNA-expressing AAVs, compared to neurons treated with a non-targeting control. (n=4, Student’s t-test, **p <0.01, ***p <0.001). (E) Ppp3r1 mRNA induction by KCl-induced depolarisation (20mM, 3h) in primary cortical neurons is inhibited by simultaneous knockdown of Piwil1 and Piwil2 (n=3–5, one way ANOVA, F (3, 10) = 8.101, **p <0.01). (F) Simultaneous knockdown of Piwil1 and Piwil2 eliminates activity-induced ING1 occupancy at the Ppp3r1 upstream regulatory region under KCl-induced depolarisation (n=3–4, one way ANOVA, F (3, 9) = 4.556, *p <0.05). Con = scrambled non-targeting shRNA. Data represent mean ± SEM.