Fig. 1.

Generation of UL16-binding protein 1 targeted pigs using CRISPR-Cas9 technology. a Schematic representation of nucleotide sequences between the target locus (exon 2 of ULBP1) and ULBP1—targeting sgRNAs. The black arrows indicate the putative cleavage site. The primers used are shown above the sequence of ULBP1. b Direct sequencing of PCR products from +/+ ULBP1, −/− ULBP1 pig #184 and alleles separated by cloning. c List of mutations. The inserted base is marked in red. Black bars indicate deletion of bases. PAM-1 and PAM-2, protospacer adjacent motifs