Fig. 3.

Expression analysis of defense-related genes in OsWRKY11-ox and OsWRKY11-kd lines. a Total RNA was isolated from WT plants and OsWRKY11-ox plants either infected with Xoo or non-infected. RT-PCR was performed using gene-specific primers for CHITINASE 2 (CHIT2), PR10, and Betv1. b OsWRKY11-kd plants were infected with Xoo using the toothpick inoculation method, and samples were collected at 24 hpi. Expression patterns of CHIT2, PR10, and Betv1 in OsWRKY11-kd lines were analyzed using RT-PCR with gene-specific primers. Transcript levels of OsACTIN were used as internal controls. These experiments were repeated more than twice